Antihyperglycemic Potential of Ethanolic Extract of Couroupita guianensis on Streptozocin Induced Experimental Diabetic Rat Model
Md. Mynul Hassan
Department of Biotechnology and Genetic Engineering, Khulna University, Khulna, Bangladesh.
Md. Mahmodul Islam *
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali, Bangladesh and Department of Pharmacy, Daffodil International University, Dhanmondi, Dhaka, Bangladesh.
Shihab Uddin
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Bangladesh.
Amrita Bhowmik
Department of Applied Laboratory Sciences, Bangladesh University of Health Sciences (BUHS), Dhaka, Bangladesh.
Begum Rokeya
Department of Pharmacology, Bangladesh University of Health Sciences (BUHS), Dhaka, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
Background: The present study was performed to investigate the effect of oral feeding of 50% ethanolic (v/v) extract of Couroupita guianensis leaves in streptozotocin induced type 2 diabetic model rats for the possible anti-diabetic mechanism.
Materials and Methods: Water, glibenclamide and extracts of Couroupita guianensis were fed to the corresponding type 2 diabetic model at the given dose (1.25 g/kg bw) for 28 consecutive days to investigate the effect on serum glucose, lipids, insulin and liver glycogen contents.
Results: The study did not cause any significant effect on degradation of depot fat, which is a common phenomenon in diabetes mellitus. Meanwhile, C. guianensis, extract improves postprandial glucose level (12.35±2.59 mmol/L) compared to control group. The serum insulin level did not increase (p= 0.148) when compared to control. Serum cholesterol level was found to be lowered at 9%. Hence, the most beneficial effect was found regarding atherogenic serum LDL level which was deceased by 2% in C. guianensis extract on 28th day. Also, the hepatic glycogen content was increased by 8% in C. guianensis extract, compared to control.
Conclusion: C. Guianensis extract has significant repressing effect on carbohydrate digestive enzymes which may function in lowering postprandial glucose levels.
Keywords: Diabetes, glucose, cholesterol, streptozocin, Couroupita guianensis.