Anti-inflammatory and Antimicrobial Potential of Three Natural Polyketides Isolated from Endophytic Fungus Phomopsis sp CAM212 against to Dysenteric Causing Pathogens
Marie Louise Medoua
Department of Biochemistry, Faculty of Science, Laboratory of Pharmacology and Toxicology, University of Yaoundé I, 812 Yaoundé, Cameroon and Department of Microbiology, Faculty of Science, Laboratory of Medical Microbiology, University of Yaoundé I, 812 Yaoundé, Cameroon.
Sylvain Nsangou Pechangou *
Department of Biochemistry, Faculty of Science, Laboratory of Pharmacology and Toxicology, University of Yaoundé I, 812 Yaoundé, Cameroon.
Brice Edie Enang II
Department of Biochemistry, Faculty of Science, Laboratory of Pharmacology and Toxicology, University of Yaoundé I, 812 Yaoundé, Cameroon.
Assam Assam Jean Paul
Laboratory of for Tuberculosis and Vector Control Biotechnology Centre, University of Yaounde1, Cameroon.
Emmanuel Mfotie Njoya
Department of Biochemistry, Faculty of Science, Laboratory of Pharmacology and Toxicology, University of Yaoundé I, 812 Yaoundé, Cameroon and Faculty of Health and Environmental Sciences, Centre for Quality of Health and Living, Central University of Technology, Bloemfontein, South Africa.
Frederic Nico Njayou
Department of Biochemistry, Faculty of Science, Laboratory of Pharmacology and Toxicology, University of Yaoundé I, 812 Yaoundé, Cameroon.
Paul Fewou Moundipa
Department of Biochemistry, Faculty of Science, Laboratory of Pharmacology and Toxicology, University of Yaoundé I, 812 Yaoundé, Cameroon.
*Author to whom correspondence should be addressed.
Abstract
Aims: The present work aimed to evaluate the anti-amoebic, antibacterial, and anti-inflammatory potential of three natural polyketides from Phomopsis sp. CAM212.
Study Design: Clinical isolates of E.histolytica, E.coli ATCC25922 strain, primary peritoneal mouse macrophages and three polyketides were used.
Places and Duration of Study: Laboratory of Pharmacology and Toxicology, Laboratory of Medical Microbiology, Faculty of Science, University of Yaounde 1 between May and December 2022.
Methodology: During this work, we evaluated the ability of three natural polyketides from Phomopsis sp to inhibit the growth of germs responsible for amoebic and bacillary dysentery. First, the anti-amoebic activity was carried out on clinical isolates of E. histolytica in polyxenic culture. Subsequently, we evaluated the antibacterial potential on a strain of E. coli ATCC25922. Finally, the anti-inflammatory potentials were evaluated on a primary culture of SC activated macrophages through inhibition of nitric oxide (NO) production, activation of phosphatase alcaline (ALP) and inhibition of 5-lipoxygenase (5-LOX).
Results: It emerges from this work that among compounds, phomopsinin B, presented the highest anti-amoebic potential (84.4 % inhibition after 72h) and the highest antibacterial potential (MIC=12.5µg/mL and MBC/MIC=2). Phomopsini A and phomopsini A acetate showed moderate anti amoebic and antibacterial potentials. However, all these activities remain lower than that of metronidazole and ciprofloxacin (90% of amoebic inhibition after 72h; MIC=0.72µg/mL and MBC/MIC=4). Subsequently, all tested compounds were nontoxic on primary macrophages. Phomopsinin B exhibited a great anti-inflammatory potential through the inhibition of NO production (IC50=1.72±0.91µg/mL); inihibition of 5-LOX activity (IC50=36.97±7.12µg/mL) and activation of ALP activity (IC50=0.13±0.01µg/mL) as compared to Baicalin the standard. The anti-inflammatory potential of phomopsinin A and phomopsinin A acetate were lower compared to baicalin.
Conclusion: Ultimately, among compounds tested, phomopsinin B exhibited the best anti-amoebic, antibacterial and ant-inflammatory potential similar to the respective standards within the limits of the tests carried out.
Keywords: Polyketides, anti-amoebic, antibacterial, anti-inflammatory, dysentery